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Scoparone

Name: Scoparone
Brand: Selleck Chemicals
SKU: S3613
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S3613

Scoparone (6,7-dimethoxycoumarin, Aesculetin dimethyl ether, 6,7-Dimethylesculetin, Escoparone) is a natural organic compound discovered from Chinese herb Artemisia scoparia with anticoagulant, hypolipidemic, vasorelaxant, antioxidant, and anti-inflammatory actions.

Chemical Structure

Molecular Weight: 206.19

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Quality Control

Batch: S361301 DMSO]41 mg/mL]false]Ethanol]41 mg/mL]false]Water]Insoluble]false Purity: 99.65%

Cited in Nature Medicine for its top-tier quality
COA
HPLC
SDS
Datasheet

99.65

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Solubility

In vitro
Batch:

DMSO : 41 mg/mL (198.84 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 41 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	206.19	Formula	C₁₁H₁₀O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	120-08-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	6,7-dimethoxycoumarin, Aesculetin dimethyl ether, 6,7-Dimethylesculetin, Escoparone			Smiles	COC1=C(C=C2C(=C1)C=CC(=O)O2)OC

Mechanism of Action

In vitro	The proliferation of VSMCs is significantly attenuated by scoparone in a dose-dependent manner. Scoparone markedly reduces the serum-stimulated accumulation of cells in the S phase and concomitantly increases the proportion of cells in the G0/G1 phase, which is consistent with the reduced expression of cyclin D1, phosphorylated Rb and survivin in the VSMCs. Cell adhesion markers, such as MCP-1 and ICAM-1, are significantly reduced by scoparone. It attenuates the increase in cyclin D promoter activity by inhibiting the activities of both the WT and active forms of STAT3. scoparone blocks the accumulation of STAT3 transported from the cytosol to the nucleus, leading to the suppression of VSMC proliferation through G1 phase arrest and the inhibition of Rb phosphorylation. This activity occurs independent of the form of STAT3 and upstream of kinases, such as Jak and Src, which are correlated with abnormal vascular remodeling due to the presence of an excess of growth factors following vascular injury. The protective effect of scoparone involves anti-inflammatory functions that occur via the inhibition of NF-κB activity, leading to the downregulation of pro-inflammatory cytokines and chemokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1.
In vivo	The free radical scavenging property of scoparone leads to the attenuation of intima thickening and improves hemodynamics in cholesterol-fed alloxan-diabetic rabbits concurrent with decreases in plasma lipid and lipoprotein cholesterol concentrations. Scoparone exhibited therapeutic efficacies on liver damage in vivo.
References	[1] https://pubmed.ncbi.nlm.nih.gov/25744297/ [2] https://pubmed.ncbi.nlm.nih.gov/25341677/

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